Table 3.

Determination of HMO in pilot plant or commercial formulae.

Sample	2′-FL (g/100 g)			LNnT (g/100 g)
	Target	HILIC	HPAEC	Target	HILIC	HPAEC
Formula (1): Intact Protein	0.800	n/a (a)	0.625	0.400	0.452 (a)	0.329
Formula (2): Intact protein	0.800	n/a (a)	0.611	0.400	0.437 (a)	0.331
Formula (3): Intact protein	0.800	n/a (a)	0.630	0.400	0.379 (a)	0.286
Formula (4): Intact protein	0.800	n/a (a)	0.637	0.400	0.394 (a)	0.288
Formula (5): Intact Protein	0.800	0.822	0.904	0.400	0.402	0.410
Formula (6): Intact Protein	0.800	0.824	0.914	0.400	0.416	0.412
Formula (7): Partially hydrolyzed protein	0.190	0.173	0.141	0	nd (b)	nd (b)
Formula (8): Intact protein	Unk. (c)	0.172	0.147	0	nd (b)	nd (b)
Formula (9): Intact protein	Unk. (c)	0.170	0.123	0	nd (b)	nd (b)
Formula (10): Partially hydrolyzed protein	Unk. (c)	0.708	0.664	Unk.	0.342	0.294

^(a) Determined using original GOS method [20] in which 2′-FL co-eluted with the internal standard, thus 2′-FL cannot be determined & LNnT was determined using only an external standard. n/a = not applicable. ^(b) nd = not detected ^(c) Unk. = Target concentration is unknown.