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. 2018 Oct 16;23(10):2650. doi: 10.3390/molecules23102650

Table 4.

Gradient for determination of 2′-FL and LNnT by HPAEC-PAD.

Time (min) Sodium Hydroxide (mM) Sodium Acetate (mM)
0 50 0
2.0 50 0
18.0 110 0
18.1 110 12.5
25.0 110 12.5
25.1 150 500
30.0 150 500
30.1 300 0
35.0 300 0
35.1 50 0
40.0 50 0