Table 1.
Antioxidant activity of daphnetin derivatives tested in 2,2′-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azinobis-(3-ethylbenzthiazoline-6-sulfonate) cation (ABTS+) assays.DAP = daphnetin.
| Compound | DPPH (EC50/μM) | ABTS+ (EC50/μM) |
|---|---|---|
| Trolox | 53.16 ± 2.02 ab | 241.33 ± 2.52 k |
| DAP (1) | 46.20 ± 1.45 ab | 159.34 ± 4.63 fghi |
| 4-Me DAP (2) | 85.63 ± 2.13 c | 224.50 ± 1.94 ijk |
| 4-Ph DAP (3) | 55.60 ± 0.78 ab | 198.33 ± 28.18 ijk |
| 4-CCH3 DAP (4) | 65.50 ± 2.84 bc | 234.68 ± 2.03 k |
| 4-CF3 DAP (5) | 45.69 ± 1.26 ab | 75.23 ± 1.58 ab |
| 4-CH2Cl DAP (6) | 46.57 ± 2.57 ab | 107.39 ± 6.72 abcde |
| 4-CH2OH DAP (7) | 61.99 ± 1.43 bc | 184.94 ± 2.04 hij |
| 4-CH2N3 DAP (8) | 62.17 ± 3.73 bc | 238.31 ± 4.47 k |
| 4-CH2COOH DAP (9) | 31.38 ± 0.68 a | 72.31 ± 2.70 a |
| 4-CH2COOMe DAP (10) | 50.20 ± 1.12 ab | 222.70 ± 1.84 jk |
| Fraxetin (11) | 45.59 ± 1.48 ab | 173.07 ± 4.83 ghi |
| Ac DAP (12) | 698.04 ± 5.22 f | 4562.49 ± 50.1 l |
| 3-Ph DAP (13) | 43.67 ± 2.08 ab | 108.01 ± 1.42 abcde |
| 3-CN DAP (14) | 47.02 ± 2.05 ab | 95.80 ± 4.18 abcd |
| 3-Ac DAP (15) | 50.62 ± 0.61 ab | 80.52 ± 0.61 abc |
| 3-COOH DAP (16) | 37.67 ± 1.16 ab | 145.67 ± 5.77 efgh |
| 3-COOEt DAP (17) | 43.98 ± 0.89 ab | 138.88 ± 0.14 defg |
| 3-PPA DAP (18) | 55.65 ± 1.48 ab | 91.25 ± 2.29 abcd |
| 3-BT DAP (19) | 71.31 ± 2.40 bc | 86.57 ± 3.17 abcd |
| 3-BT-4-COOH DAP (21) | 130.50 ± 3.54 d | 108.33 ± 6.35 abcde |
| 3-BT-4-CONH2 DAP (22) | 66.82 ± 1.38 bc | 122.34 ± 4.41 bcdef |
| 3-BT-4-CN4H DAP (23) | 167.85 ± 11.63 e | 121.72 ± 0.22 cdef |
| 4-CF3-3-Me DAP (24) | 53.76 ± 2.25 ab | 107.21 ± 6.23 abcde |
Values marked by the same alphabet are not significantly different (p < 0.05).