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. 2018 Jul 26;23(8):1857. doi: 10.3390/molecules23081857

Figure 1.

Figure 1

Oregano essential oil (OEO) inhibits lipopolysaccharide (LPS)-induced mRNA levels of pro-inflammatory cytokines expression in murine macrophage cells (RAW264.7 cells). (a–c) Cells were pretreated with OEO for 12 h, then incubated with 1 μg/mL LPS for 12 h. (d–f) Cells were pretreated with OEO for 12 h, then incubated with 1 μg/mL LPS for 1 h. The interleukin-1 beta (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-α) mRNA expression levels were measured using an RT-PCR analysis. Values represent means ± standard error of the mean (SEM), n = 3. # p < 0.05 and ## p < 0.01 compared to the control group, while * p < 0.05 and ** p < 0.01 compared to the LPS-treated group.