Figure 8.

Verification expression level of eight overlapped DEGs in breast cancer cell lines. (a) Real-time PCR detecting the transcription levels of eight overlapped DEGs on MDA-MB-231 cell line by gain ERα. The results were normalized to GAPDH, with the mean ± SD of three replicates. (b) MCF-7 cells were treated with estrogen (E2) and subjected to quantitative reverse transcriptase PCR (qRT-PCR) assay (n = 3). (c) Verification common genes expression by Pearson correlation analysis based on TCGA breast tumor dataset.