Figure 4.

Effects on intracellular tyrosinase activity in murine B16-F10 cells; B16-F10 cells were pretreated with test samples for 2 h and then incubated with α-MSH (200 nM) for 72 h. The cell lysates (50 μg) were dissolved in 100 mM phosphate buffer (pH 6.8) and treated with L-dopa (2 mg/mL) in a 96-well plate at 37 °C for 2 h. The production amount of dopachrome was measured by using an ELISA microplate reader at 490 nm. * p < 0.05 and *** p < 0.001 compared with the α-MSH-treated group.