Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Oct 8;23(10):2559. doi: 10.3390/molecules23102559

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Effects on melanogenesis-related protein expression in α-MSH-induced murine B16-F10 cells; B16-F10 cells were pretreated with test samples for 2 h and α-MSH was added to the cells for 24 h. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as the control protein. The protein levels of microphthalmia-associated transcription factor (MITF), tyrosinase, tyrosinase-related protein-1 (TRP-1), and tyrosinase-related protein-2 (TRP-2) were detected by Western blot analysis. Compounds 3 and 8 significantly reduced the expression of MITF, tyrosinase, TRP-1, and TRP-2 in α-MSH-induced B16-F10 cells. Arbutin, a positive control drug, also blocked the melanogenesis. (A) MITF, (B) TRP-1, (C) TRP-2, and (D) tyrosinase were analyzed and quantified using Azure Spot software. MITF, TRP-1, TRP-2 and tyrosinase protein levels were normalized by GAPDH.

Effects on melanogenesis-related protein expression in α-MSH-induced murine B16-F10 cells; B16-F10 cells were pretreated with test samples for 2 h and α-MSH was added to the cells for 24 h. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as the control protein. The protein levels of microphthalmia-associated transcription factor (MITF), tyrosinase, tyrosinase-related protein-1 (TRP-1), and tyrosinase-related protein-2 (TRP-2) were detected by Western blot analysis. Compounds 3 and 8 significantly reduced the expression of MITF, tyrosinase, TRP-1, and TRP-2 in α-MSH-induced B16-F10 cells. Arbutin, a positive control drug, also blocked the melanogenesis. (A) MITF, (B) TRP-1, (C) TRP-2, and (D) tyrosinase were analyzed and quantified using Azure Spot software. MITF, TRP-1, TRP-2 and tyrosinase protein levels were normalized by GAPDH.