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. 2018 Oct 8;23(10):2559. doi: 10.3390/molecules23102559

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effects of M. alba leaves on the phosphorylation of the cAMP-responsive element-binding protein (CREB) and p38 in α-MSH-induced B16-F10 cell; B16-F10 cells were pretreated with test samples for 2 h and α-MSH was added to the cells for 24 h. Total protein was used as the control protein. The protein levels of pCREB, CREB, pp38, p38 were detected by Western blot analysis. Compound 3 significantly reduced the expression of pCREB and pp38 in α-MSH-induced B16-F10 cells. Arbutin, a positive control drug, also blocked the melanogenesis. The protein level of (A) pCREB and (B) pp38 were quantitated with Azure Spot software. pCREB and pp38 protein levels were normalized by the total protein of CREB or p38.