Figure 2.

10j induces simultaneous blockage of both HCV replication and particle production in a mechanistically separate manner (A) Huh7.5-J6/JFH1 cells were treated with an increasing concentration of 10j for a designated period of time (3, 6, 12, 24, 48, and 72 h). Then, their total RNAs were extracted and intracellular HCV RNA levels were measured by a real-time RT-PCR analysis. (B) In parallel, supernatants collected from 10j-treated Huh7.5-J6/JFH1 cells were incubated with naïve Huh7.5 cells to measure its infectivity by another round of a real-time RT-PCR analysis. (C) EC₅₀ values on HCV genome replication and particle productions were calculated based on data shown in (A,B). (D,E) Same experiments were performed as in (A,B) by using A922500 except 3 h incubation. (F) EC₅₀ values on HCV genome replication and particle productions were calculated based on data shown in (D,E).