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. 2018 Aug 20;23(8):2083. doi: 10.3390/molecules23082083

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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10j inhibits HCV replication in a reporter-free system. (A) Dose- as well as (B) time-dependent effects of 10j on genotype 2a HCV genome replication was determined by measuring relative HCV as well as GAPDH RNA levels via a real-time RT-PCR analysis of Huh7.5-J6/JFH1 cells (C) Dose- as well as (D) time-dependent effects of 10j on genotype 1b HCV genome replication was determined by measuring relative HCV as well as GAPDH RNA levels via a real-time RT-PCR analysis of Huh7.5-Bart79I cells. (E) Huh7.5-J6/JFH1 cells were treated with a series of increasing concentrations of 10j for 72 h. Then, cells were stained with DAPI in blue for nucleus, an anti-J2 antibody in green for dsRNA and Oil-red-O in red for LD. (F) Quantitation of dsRNA and Oil red O signals shown in the Figure 3E.