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. 2018 Aug 20;23(8):2083. doi: 10.3390/molecules23082083

Figure 7.

Figure 7

(A) Huh7.5-J6/JFH1 cells were treated with increasing concentrations of 10j for 72 h. Cells were stained with an anti-NS3 antibody in red, DAPI in blue for nucleus and BODIPY for LD. (B) Relative percentages of NS3-and BODIPY-positive cells were quantified from more than three images from experiments shown in Figure 6A. (C) Huh7.5-JFH1-5A-GFP cells were treated with increasing concentrations of 10j for 72 h. Cells were visualized in green for NS5A, DAPI in blue for nucleus and Oil Red O for LD. (D) Relative percentages of NS3-GFP- and LD-positive cells were quantified from more than three images from experiments shown in Figure 6C. (E) Effect of 10j on HCV IRES-dependent translation. Huh7.5 cells were transfected with HCV IRES luciferase reporter plasmid followed by treatment of increasing concentrations of 10j. HCV IRES-dependent translation efficiency was measured by a luciferase assay.