Table 2.

Precision and accuracy of the determination of the 10 alkaloids in the beagle dog plasma (n = 18, six replicates per day for three days).

Compound	Spiked Concentration (ng/mL)	Measured Concentration (ng/mL)	Accuracy (RE%)	Intra-Day Precision (RSD%)	Inter-Day Precision (RSD%)
corydaline	0.5	0.53 ± 0.03	−7.50	10.90	6.00
	1.1	0.96 ± 0.07	−10.75	8.22	2.53
	34.8	32.87 ± 1.69	−5.56	5.18	4.82
	445.6	432.80 ± 18.00	−2.86	3.99	5.24
dehydrocorydaline	0.3	0.21 ± 0.04	−12.10	12.10	12.40
	0.5	0.43 ± 0.05	−4.95	4.43	8.30
	16.1	14.59 ± 1.45	−9.34	9.94	10.13
	206.0	196.20 ± 8.40	−4.75	3.81	6.79
tetrahydropalmatine	0.5	0.45 ± 0.03	−6.90	8.80	12.10
	1.0	0.93 ± 0.05	−5.82	5.00	7.95
	31.7	30.04 ± 1.50	−5.18	5.19	2.72
	405.6	401.50 ± 20.26	−3.84	4.15	4.22
protopine	0.5	0.48 ± 0.02	−11.90	9.20	8.80
	1.1	0.94 ± 0.04	−5.96	4.97	1.53
	33.8	31.54 ± 0.88	−6.56	2.76	2.96
	432.0	403.30 ± 14.58	−6.64	3.61	3.64
palmatine	0.5	0.47 ± 0.02	−9.60	7.00	12.00
	1.0	0.91 ± 0.11	−12.32	10.51	7.09
	32.8	30.56 ± 1.48	−6.83	5.02	3.42
	420.0	401.90 ± 12.13	−4.29	2.94	3.54
tetrahydroberberine	0.4	0.39 ± 0.03	−12.90	7.79	7.30
	0.8	0.78 ± 0.06	−4.46	7.57	4.67
	26.3	24.17 ± 1.80	−7.92	7.66	5.50
	336.0	306.40 ± 24.86	−8.81	8.02	8.80
columbamine	0.4	0.38 ± 0.02	−10.50	11.40	13.70
	0.8	0.76 ± 0.04	−3.19	5.04	4.61
	25.0	24.02 ± 1.41	−3.92	6.19	2.35
	320.0	295.20 ± 7.17	−7.74	2.16	3.91
berberine	0.5	0.42 ± 0.05	−14.40	11.20	14.90
	1.1	0.87 ± 0.09	−13.22	10.66	3.28
	33.8	32.04 ± 0.74	−5.07	2.40	1.35
	432.0	411.60 ± 17.07	−4.73	4.22	3.60
coptisine	0.5	0.47 ± 0.04	−5.90	8.80	13.40
	1.0	0.92 ± 0.06	−7.71	6.13	7.29
	31.3	30.28 ± 0.89	−3.10	2.78	3.97
	400.0	389.40 ± 9.20	−2.66	2.30	2.78
berberrubine	0.2	0.18 ± 0.04	−16.09	19.40	18.20
	0.5	0.41 ± 0.02	−8.70	5.93	6.70
	14.4	13.48 ± 1.15	−6.18	7.89	12.47
	184.0	185.50 ± 10.41	0.83	5.15	8.30