Table 3.
IS-normalized matrix factor and extraction recovery for the 10 alkaloids in the beagle dog plasma. (n = 6).
| Compound | Spiked Concentration (ng/mL) | IS-Normalized Matrix Factor | Extraction Recovery | |
|---|---|---|---|---|
| Mean (%) | RSD (%) | |||
| corydaline | 1.1 | 0.91 | 79.36 | 9.903 |
| 34.8 | 0.97 | 85.91 | 6.930 | |
| 445.6 | 1.00 | 79.11 | 9.320 | |
| dehydrocorydaline | 0.5 | 1.00 | 84.12 | 8.781 |
| 16.1 | 1.04 | 88.02 | 8.180 | |
| 206.0 | 1.14 | 86.84 | 5.280 | |
| tetrahydropalmatine | 1.0 | 0.94 | 90.72 | 9.730 |
| 31.7 | 0.98 | 87.25 | 12.68 | |
| 507.0 | 1.01 | 87.96 | 10.15 | |
| protopine | 1.1 | 1.15 | 84.33 | 12.67 |
| 33.8 | 1.08 | 91.22 | 13.75 | |
| 432.0 | 1.09 | 83.46 | 4.350 | |
| palmatine | 1.0 | 0.94 | 91.21 | 11.01 |
| 32.8 | 0.87 | 87.29 | 7.730 | |
| 420.0 | 0.74 | 84.82 | 7.230 | |
| tetrahydroberberine | 0.8 | 0.89 | 89.17 | 12.30 |
| 26.3 | 0.88 | 88.48 | 5.243 | |
| 336.0 | 0.92 | 93.00 | 8.870 | |
| columbamine | 0.8 | 0.96 | 96.58 | 11.31 |
| 25.0 | 0.91 | 91.61 | 11.58 | |
| 320.0 | 0.94 | 84.66 | 5.910 | |
| berberine | 1.1 | 0.90 | 90.06 | 6.380 |
| 33.8 | 0.94 | 84.58 | 5.520 | |
| 432.0 | 0.97 | 87.94 | 7.270 | |
| coptisine | 1.0 | 0.95 | 91.75 | 9.640 |
| 31.3 | 0.88 | 88.93 | 7.880 | |
| 400.0 | 0.95 | 85.60 | 6.890 | |
| berberrubine | 0.5 | 0.94 | 88.41 | 7.790 |
| 14.4 | 0.97 | 77.09 | 12.86 | |
| 184.0 | 0.91 | 98.55 | 5.000 | |