Figure 3.

CR retards age‐associated increases in collagen deposition within the aortic walls. A, Representative photomicrographs of paraffin thoracic aortic sections of both young and old F344 rats with an AL or a CR diet stained with Sirius Red for collagen (red), observed using conventional light microscopy (×200). n=5 rats/group, respectively. Bar scale, 20 μm. B, Collagen density shown as mean±SEM (n=5 animals/group). The data were analyzed with a 2‐way ANOVA: P<0.05 for age×diet interaction. Pairwise comparison: old vs young within AL group and AL vs CR in old group were shown (*P<0.05). AL indicates ad libitum; CR, calorie restriction; L, lumen; M, media.