Table 1.
Primary Antibodies
| Antibody | Species | Titer Blotting | Titer Staining | Sources |
|---|---|---|---|---|
| α‐SMA | M | 1:1000 | 1:20 | Dako Corp, Denmark |
| CD31 | M | 1:50 | Dako Corp, Denmark | |
| MT1‐MMP | R, M | 1:250 | Chemicon Intern. Inc, CA | |
| TIMP2 | R, M | 1:100 | Chemicon Intern. Inc, CA | |
| LTBP‐1 | M | 1:50 | Chemicon Intern. Inc, CA | |
| LAP | M | 1:50 | Chemicon Intern. Inc, CA | |
| p‐SMAD2/3 | R | 1:1000 | 1:50 | Cell signaling, CA |
| SMAD2/3 | R | 1:1000 | Cell signaling, CA | |
| Collagen I | R | 1:1000 | 1:50 | Rockland Immunochemicals, Inc, PA |
| TGF‐β1 | R | 1:200 | 1:50 | Santa Cruz, CA |
| SM22α | G | 1:2000 | Abcam, MA | |
| Myocardin | M | 1:1000 | Abcam, MA | |
| β‐actin | M | 1:10 000 | 1:50 | Sigma‐Aldrich, Inc, MO |
CD31 indicates cluster of differentiation 31; G, goat; LAP, latent associated protein; LTBP‐1, latent TGF binding protein‐1; M, mouse; MT1‐MMP, membrane type 1 matrix metalloproteinase; p‐SMAD2/3, phospho‐mothers against decapentaplegic homolog‐2/3; R, rabbit; SMAD2/3, mothers against decapentaplegic homolog‐2/3; TGF‐β1, transforming growth factor beta 1; TIMP2, tissue inhibitor of metalloproteinases 2; α‐SMA, alpha smooth muscle actin.