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. 2018 Sep 17;7(18):e008604. doi: 10.1161/JAHA.118.008604

Figure 2.

Figure 2

Cardiac ADAM23 deficiency exacerbates aortic banding (AB)‐induced hypertrophy. A, The ratios of heart weight to tibia length (HW/TL) and heart weight to body weight (HW/BW) were determined in the indicated groups 4 weeks after AB surgery (n=13 mice for AFMC sham group, n=12 mice for cADAM23‐KO sham group and AFMC AB group, n=11 mice for cADAM23‐KO AB group). B, Statistical results for the echocardiographic parameters (LVEDd [top] and FS [bottom]) in the indicated groups (n=12 mice for AFMC sham group and AFMC AB group, n=11 mice for cADAM23‐KO sham group and cADAM23‐KO AB group). C, Histological analysis of the whole hearts (left) and heart sections stained with H&E (right) from the indicated groups 4 weeks after sham or AB surgery (n=6 mice per group; scale bar, 1 mm). D, Representative images of heart sections stained with H&E and WGA staining, and statistical results for the cardiomyocyte CSA (n>100 cells per group; scale bar, 20 μm). E, Representative images of histological analysis of cardiac perivascular and interstitial fibrosis, and statistical results for the fibrotic area (n>40 fields per group; scale bar, 20 μm). F, Quantification results for mRNA levels of the hypertrophic marker genes (Anp, Bnp and β‐Mhc) and fibrotic marker genes (collagen I, collagen III, and Ctgf) in the indicated groups (n=4 independent experiments). AB indicates aortic banding; ANP, atrial natriuretic peptide; CSA, cross‐sectional area; DCM, dilated cardiomyopathy; FS, fractional shortening; H&E, hematoxylin and eosin; HCM, hypertrophic cardiomyopathy; HW/BW, heart weight to body weight; HW/TL, heart weight to tibia length; KO, knockout; LVEDd, left ventricular end‐diastolic diameter, WGA, wheat germ agglutinin. *P<0.05 vs sham; # P<0.05 vs AFMC AB group in (A, B, D through F).