Fig. 1.

The construction of experimental chamber in clean room and H₂O₂ concentrations. (a) Schematic illustration of the experimental environments. An experimental chamber with vinyl sheet was set in a biological clean room including a BSC and H₂O₂ generator. Filled squares indicate H₂O₂ sensors, and the dotted line indicates the vinyl sheet. (b) Time profile of H₂O₂ concentration. Curved line shows the concentration of H₂O₂ in the vinyl sheet measured by the sensor A in Fig. 1 during the decontamination. (c) Line shows the concentration of H₂O₂ in the BSC measured by sensor B and c in Fig. 1 during and after decontamination. The minimum detection limit of the sensors was 3 mg/L. Time A: t = 0 is defined as the time point at which H₂O₂ concentration reached 1 mg/L, as measured by a detection tube. The dotted line indicates the end of 5 h of decontamination. Each arrow indicates the time when the respective dish was uncovered in the subsequent experiments.