Table 6.
Quantitative analysis of PCs in layer 6 of rat SSC.
| L6_TPC:A | L6_TPC:C | L6_UPC | L6_IPC | L6_BPC | L6_HPC |
t-test |
||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| (n = 26) | (n = 18) | (n = 23) | (n = 27) | (n = 32) | (n = 7) | TPC:A vs. TPC:C | TPC:A vs. UPC | TPC:A vs. IPC | TPC:A vs. BPC | UPC vs. TPC:C | IPC vs. TPC:C | BPC vs.TPC:C | UPC vs. IPC | UPC vs. BPC | IPC vs.BPC | HPC vs TPC:A | HPC vs TPC:C | HPC vs UPC | HPC vs IPC | HPC vs BPC | ||
| Soma | Perimeter(μm) | 53 ± 2 | 51±3 | 51 ±1 | 54 ±2 | 53 ±2 | 64±3 | ns | ns | ns | ns | ns | ns | ns | ns | ns | ns | < 0.05 | < 0.05 | < 0.05 | < 0.05 | < 0.05 |
| Area(μm²) | 184 ±12 | 159 ±9 | 179 ±8 | 179 ±9 | 185 ±13 | 243 ±10 | ns | ns | ns | ns | ns | ns | ns | ns | ns | ns | < 0.05 | < 0.05 | < 0.05 | < 0.05 | < 0.05 | |
| Basal Dendrites | Max horizontal extend (μm) | 217 ±14 | 161 ±7 | 232±15 | 248±26 | 196 ±13 | 407 ±85 | < 0.05 | ns | ns | ns | < 0.05 | < 0.05 | < 0.05 | ns | ns | ns | < 0.05 | < 0.05 | ns | ns | < 0.05 |
| Max vertical extend (μm) | 208 ±16 | 123 ±7 | 198 ±15 | 192 ±18 | 171 ±18 | 360 ±51 | < 0.05 | ns | ns | ns | < 0.05 | < 0.05 | < 0.05 | ns | ns | ns | < 0.05 | < 0.05 | < 0.05 | < 0.05 | < 0.05 | |
| Den# | 5 ±0.3 | 7 ±0.4 | 6 ±0.3 | 6 ±0.3 | 4 ±0.3 | 4 ±0.7 | < 0.05 | ns | ns | < 0.05 | ns | < 0.05 | < 0.05 | ns | < 0.05 | < 0.05 | < 0.05 | < 0.05 | < 0.05 | < 0.05 | ns | |
| Length(μm) | 1716±128 | 1257±92 | 1857±188 | 1522 ±111 | 1174 ±103 | 2029 ±395 | < 0.05 | ns | ns | < 0.05 | < 0.05 | ns | ns | ns | < 0.05 | < 0.05 | ns | ns | ns | ns | ns | |
| Surface(μm²) | 3039 ±282 | 2150±200 | 3500±373 | 2540±207 | 2063 ±221 | 4444 ±1320 | < 0.05 | ns | ns | < 0.05 | < 0.05 | ns | ns | < 0.05 | < 0.05 | ns | ns | ns | ns | ns | ns | |
| Volume(μm³) | 567±71 | 360 ±61 | 699 ±98 | 464±63 | 385 ±59 | 1145 ±469 | < 0.05 | ns | ns | < 0.05 | < 0.05 | ns | ns | < 0.05 | < 0.05 | ns | ns | ns | ns | ns | ns | |
| Tree length (μm) | 349 ±34 | 203 ±20 | 338±32 | 299 ±38 | 299 ±25 | 726 ±197 | < 0.05 | ns | ns | ns | < 0.05 | < 0.05 | < 0.05 | ns | ns | ns | ns | < 0.05 | ns | ns | ns | |
| Seg length (μm) | 56 ±4 | 45 ±3 | 64 ±6 | 63 ±6 | 56 ±3 | 101±8 | < 0.05 | ns | ns | ns | < 0.05 | < 0.05 | < 0.05 | ns | ns | ns | < 0.05 | < 0.05 | < 0.05 | < 0.05 | < 0.05 | |
| Seg surface (μm²) | 96 ±7 | 80 ±9 | 127±17 | 103 ±8 | 100 ±7 | 212 ±32 | ns | ns | ns | ns | < 0.05 | ns | ns | ns | ns | ns | < 0.05 | < 0.05 | < 0.05 | < 0.05 | < 0.05 | |
| Seg volume (μm³) | 18 ±2 | 14 ±3 | 27 ±6 | 19 ±3 | 19 ±3 | 52 ±13 | ns | ns | ns | ns | < 0.05 | ns | ns | ns | ns | ns | < 0.05 | < 0.05 | ns | < 0.05 | < 0.05 | |
| Seg# | 34 ±4 | 28 ±2 | 33 ±5 | 27 ±2 | 22 ±2 | 20 ±3 | ns | ns | ns | < 0.05 | ns | ns | < 0.05 | ns | ns | ns | < 0.05 | < 0.05 | < 0.05 | ns | ns | |
| Tortuosity | 1.27±0.01 | 1.34 ±0.03 | 1.28±0.02 | 1.25 ±0.01 | 1.27 ±0.02 | 1.20 ±0.02 | < 0.05 | ns | ns | ns | ns | < 0.05 | ns | ns | ns | ns | < 0.05 | < 0.05 | < 0.05 | < 0.05 | < 0.05 | |
| Max order | 5±0.6 | 4±0.2 | 5±0.3 | 4±0.2 | 5±0.5 | 4±0.5 | ns | ns | < 0.05 | ns | ns | ns | ns | ns | ns | ns | ns | ns | ns | ns | ns | |
| Mean order | 3±0.3 | 3±0.2 | 3±0.2 | 3±0.2 | 3±0.2 | 3±0.5 | < 0.05 | ns | < 0.05 | ns | ns | ns | ns | ns | ns | ns | ns | ns | ns | ns | ns | |
| Max angles | 61 ±2 | 64 ±2 | 57 ±2 | 60 ±2 | 57 ±2 | 46±2 | ns | ns | ns | ns | < 0.05 | ns | < 0.05 | ns | ns | ns | < 0.05 | < 0.05 | < 0.05 | < 0.05 | < 0.05 | |
| Planar angle | 44 ±1 | 42 ±4 | 42 ±1 | 42 ±2 | 38 ±2 | 34 ±1 | ns | ns | ns | < 0.05 | ns | ns | ns | ns | ns | ns | < 0.05 | < 0.05 | < 0.05 | < 0.05 | ns | |
| Local angle | 58 ±2 | 51 ±6 | 58 ±2 | 56 ±2 | 58 ±3 | 50 ±3 | ns | ns | ns | ns | ns | ns | ns | ns | ns | ns | < 0.05 | ns | < 0.05 | < 0.05 | < 0.05 | |
| Local spline angle | 54 ±2 | 47 ±5 | 50 ±1 | 48±2 | 50 ±2 | 43 ±3 | ns | < 0.05 | < 0.05 | ns | ns | ns | ns | ns | ns | ns | < 0.05 | ns | < 0.05 | ns | < 0.05 | |
| Apical Dendrites | Max horizontal extend (μm) | 321 ±20 | 189 ±11 | 249 ±19 | 312 ±22 | 219±17 | 431±83 | < 0.05 | < 0.05 | ns | < 0.05 | < 0.05 | < 0.05 | ns | < 0.05 | ns | < 0.05 | ns | < 0.05 | ns | ns | < 0.05 |
| Max vertical extend (μm) | 690±33 | 731 ±53 | 714±48 | 397 ±32 | 580 ±36 | 451 ±62 | ns | ns | < 0.05 | < 0.05 | ns | < 0.05 | < 0.05 | < 0.05 | < 0.05 | < 0.05 | < 0.05 | < 0.05 | < 0.05 | ns | ns | |
| Length(μm) | 3390 ±194 | 3239±210 | 3136±280 | 2612±255 | 2304±191 | 3215 ±912 | ns | ns | < 0.05 | < 0.05 | ns | ns | < 0.05 | ns | < 0.05 | ns | ns | ns | ns | ns | ns | |
| Surface(μm²) | 6120±456 | 6413±597 | 6242±605 | 4797±434 | 3955 ±283 | 6194 ±1206 | ns | ns | < 0.05 | < 0.05 | ns | < 0.05 | < 0.05 | ns | < 0.05 | ns | ns | ns | ns | ns | ns | |
| Volume(μm³) | 1192±142 | 1403±245 | 1425±200 | 979 ±98 | 766 ±83 | 1472 ±289 | ns | ns | ns | < 0.05 | ns | ns | < 0.05 | ns | < 0.05 | ns | ns | ns | ns | ns | < 0.05 | |
| Seg length (μm) | 90 ±6 | 62 ±4 | 82 ±7 | 71 ±6 | 78 ±6 | 98 ±5 | < 0.05 | ns | < 0.05 | ns | < 0.05 | ns | < 0.05 | ns | ns | ns | ns | < 0.05 | ns | < 0.05 | < 0.05 | |
| Seg surface (μm²) | 160 ±11 | 122±10 | 167 ±17 | 138 ±11 | 151 ±20 | 204 ±23 | < 0.05 | ns | ns | ns | < 0.05 | ns | ns | ns | ns | ns | ns | < 0.05 | ns | < 0.05 | ns | |
| Seg volume (μm³) | 31 ±4 | 26 ±4 | 38 ±5 | 31 ±4 | 32 ±6 | 51 ±10 | ns | ns | ns | ns | ns | ns | ns | ns | ns | ns | ns | < 0.05 | ns | ns | ns | |
| Seg# | 43 ±5 | 54 ±5 | 42 ±5 | 39 ±4 | 34 ±4 | 31±7 | ns | ns | ns | ns | ns | < 0.05 | < 0.05 | ns | ns | ns | ns | < 0.05 | ns | ns | ns | |
| Tortuosity | 1.22 ±0.02 | 1.28±0.02 | 1.25 ±0.03 | 1.22 ±0.03 | 1.23±0.01 | 1.20 ±0.03 | ns | ns | ns | ns | ns | ns | ns | ns | ns | ns | ns | < 0.05 | ns | ns | ns | |
| Max order | 14 ±1 | 20±1 | 14 ±1 | 12 ±1 | 12 ±1 | 7 ±1 | < 0.05 | ns | ns | < 0.05 | < 0.05 | < 0.05 | < 0.05 | ns | < 0.05 | ns | < 0.05 | < 0.05 | < 0.05 | < 0.05 | < 0.05 | |
| Max angles | 59 ±2 | 66 ±2 | 63±2 | 61 ±2 | 55 ±2 | 54 ±4 | < 0.05 | ns | ns | ns | ns | < 0.05 | < 0.05 | ns | < 0.05 | < 0.05 | ns | < 0.05 | ns | ns | ns | |
| Planar angle | 39 ±1 | 40±3 | 42 ±1 | 43 ±1 | 36 ±2 | 40 ±3 | ns | ns | ns | ns | ns | ns | ns | ns | < 0.05 | < 0.05 | ns | ns | ns | ns | ns | |
| Local angle | 61 ±1 | 55 ±4 | 59 ±2 | 63 ±2 | 57 ±2 | 56 ±4 | ns | ns | ns | ns | ns | < 0.05 | ns | ns | ns | < 0.05 | ns | ns | ns | ns | ns | |
| Local spline angle | 52 ±1 | 51 ±2 | 53 ±1 | 52 ±1 | 49 ±1 | 49 ±3 | ns | ns | ns | ns | ns | ns | ns | ns | < 0.05 | ns | ns | ns | ns | ns | ns | |
| Oblique# | 9.2±0.7 | 14.4 ±0.9 | 12.9±1.0 | 11 ±1 | 8.6 ±0.6 | 3.7±0.8 | < 0.05 | < 0.05 | ns | ns | ns | < 0.05 | < 0.05 | ns | < 0.05 | < 0.05 | < 0.05 | < 0.05 | < 0.05 | < 0.05 | < 0.05 | |
| Axon | Max horizontal extend (μm) | 778±136 | 315 ±53 | 798 ±113 | 868 ±133 | 841±98 | 715 ±157 | < 0.05 | ns | ns | ns | < 0.05 | < 0.05 | < 0.05 | ns | ns | ns | ns | < 0.05 | ns | ns | ns |
| Max vertical extend (μm) | 560 ±50 | 628 ±102 | 691±87 | 750 ±90 | 691 ±56 | 773 ±247 | ns | ns | ns | ns | ns | ns | ns | ns | ns | ns | ns | ns | ns | ns | ns | |
| Length(μm) | 3648 ±545 | 2503±508 | 4670±753 | 4947 ±859 | 4428 ±570 | 4518±1480 | ns | ns | ns | ns | < 0.05 | < 0.05 | < 0.05 | ns | ns | ns | ns | ns | ns | ns | ns | |
| Surface(μm²) | 2397 ±356 | 2387 ±517 | 3074±505 | 3077±467 | 2729 ±324 | 2910±821 | ns | ns | ns | ns | ns | ns | ns | ns | ns | ns | ns | ns | ns | ns | ns | |
| Volume(μm³) | 204±33 | 254 ±62 | 286 ±48 | 220 ±36 | 227 ±40 | 251 ±88 | ns | ns | ns | ns | ns | ns | ns | ns | ns | ns | ns | ns | ns | ns | ns | |
| Seg length (μm) | 125±14 | 87 ±13 | 106±10 | 115 ±11 | 113 ±7 | 112 ±16 | < 0.05 | ns | ns | ns | ns | ns | ns | ns | ns | ns | ns | ns | ns | ns | ns | |
| Seg surface (μm²) | 108 ±19 | 91 ±14 | 76 ±8 | 89 ±15 | 74 ±8 | 79 ±13 | ns | ns | ns | ns | ns | ns | ns | ns | ns | ns | ns | ns | ns | ns | ns | |
| Seg volume (μm³) | 14±4 | 11 ±2 | 8 ±1 | 8 ±2 | 7 ±1 | 8 ±2 | ns | ns | ns | ns | ns | ns | ns | ns | ns | ns | ns | ns | ns | ns | ns | |
| Seg# | 39 ±8 | 45 ±19 | 51 ±12 | 44 ±9 | 42 ±5 | 46 ±17 | ns | ns | ns | ns | ns | ns | ns | ns | ns | ns | ns | ns | ns | ns | ns | |
| Tortuosity | 1.23±0.03 | 1.18±0.02 | 1.19 ±0.01 | 1.19 ±0.01 | 1.17±0.01 | 1.17 ±0.02 | ns | ns | ns | ns | ns | ns | ns | ns | ns | ns | ns | ns | ns | ns | ns | |
| Max order | 7 ±1 | 10 ±2 | 10 ±1 | 8 ±0.8 | 10 ±1 | 10 ±3 | ns | ns | ns | < 0.05 | ns | ns | ns | ns | ns | ns | ns | ns | ns | ns | ns | |
| Max angles | 75 ±2 | 92 ±5 | 80 ±3 | 69 ±2 | 74 ±1 | 83 ±4 | < 0.05 | ns | ns | ns | ns | < 0.05 | < 0.05 | < 0.05 | ns | ns | ns | ns | ns | ns | ns | |
| Planar angle | 50±2 | 62 ±4 | 54 ±2 | 47 ±2 | 49 ±1 | 55 ±4 | < 0.05 | ns | ns | ns | ns | < 0.05 | < 0.05 | < 0.05 | < 0.05 | ns | ns | ns | ns | ns | ns | |
| Local angle | 62 ±2 | 63 ±2 | 62 ±2 | 62 ±2 | 62 ±1 | 65 ±6 | ns | ns | ns | ns | ns | ns | ns | ns | ns | ns | ns | ns | ns | ns | ns | |
| Local spline angle | 54±1 | 56 ±2 | 53 ±1 | 51 ±1 | 55 ±1 | 60±6 | ns | ns | < 0.05 | ns | ns | < 0.05 | ns | ns | ns | < 0.05 | ns | ns | ns | ns | ns | |
| Boton density (#/100 μm) | 20±2 | 17 ±2 | 19 ±1 | 19 ±2 | 20 ±1 | 22 ±1 | ns | ns | ns | ns | ns | ns | ns | ns | ns | ns | ns | < 0.05 | ns | ns | ns | |