Table 1.
Plasmids and strains used in this study
| Name | Relevant characteristics | References |
|---|---|---|
| Plasmids | ||
| pETDuet-1 | ColE1(pBR322) ori; Ampr; PT7 | Novagen |
| pETDuet-KDC0498 | ColE1(pBR322) ori; Ampr; PT7-kdc0498 | This work |
| pETDuet-KDC0505 | ColE1(pBR322) ori; Ampr; PT7-kdc0505 | This work |
| pETDuet-KDC1244 | ColE1(pBR322) ori; Ampr; PT7-kdc1244 | This work |
| pETDuet-KDC1476 | ColE1(pBR322) ori; Ampr; PT7-kdc1476 | This work |
| pETDuet-KDC3074 | ColE1(pBR322) ori; Ampr; PT7-kdc3074 | This work |
| pETDuet-KDC3075 | ColE1(pBR322) ori; Ampr; PT7-kdc3075 | This work |
| pETDuet-KDC3076 | ColE1(pBR322) ori; Ampr; PT7-kdc3076 | This work |
| pETDuet-KDC3652 | ColE1(pBR322) ori; Ampr; PT7-kdc3652 | This work |
| pETDuet-KDC4427 | ColE1(pBR322) ori; Ampr; PT7-kdc4427 | |
| pETDuet-KDC4472 | ColE1(pBR322) ori; Ampr; PT7-kdc4472 | This work |
| pETDuet-Aro10 | ColE1(pBR322) ori; Ampr; PT7-ARO10 | This work |
| pETDuet-aroFcoli–pheAcoli | ColE1(pBR322) ori; Ampr; PT7-aroFcoli–pheAcoli | Reference [37] |
| pETDuet-aroFcoli-pheA3272 | ColE1(pBR322) ori; Ampr; PT7-aroFcoli-pheA3272 | This work |
| pETDuet-aroFcoli-pheA3270 | ColE1(pBR322) ori; Ampr; PT7-aroFcoli-pheA3270 | This work |
| pETDuet-aroG1710-pheAcoli | ColE1(pBR322) ori; Ampr; PT7-aroG1710-pheAcoli | This work |
| pETDuet-aroG1710-pheA3272 | ColE1(pBR322) ori; Ampr; PT7-aroG1710-pheA3272 | This work |
| pETDuet-aroG1710-pheA3270 | ColE1(pBR322) ori; Ampr; PT7-aroG1710-pheA3270 | This work |
| pETDuet-aroH2193-pheAcoli | ColE1(pBR322) ori; Ampr; PT7-aroH2193-pheAcoli | This work |
| pETDuet-aroH2193-pheA3272 | ColE1(pBR322) ori; Ampr; PT7-aroH2193-pheA3272 | This work |
| pETDuet-aroH2193-pheA3270 | ColE1(pBR322) ori; Ampr; PT7-aroH2193-pheA3270 | This work |
| pETDuet-aroF3269-pheAcoli | ColE1(pBR322) ori; Ampr; PT7-aroF3269-pheAcoli | This work |
| pETDuet-aroF3269-pheA3272 | ColE1(pBR322) ori; Ampr; PT7-aroF3269-pheA3272 | This work |
| pETDuet-aroF3269-pheA3270 | ColE1(pBR322) ori; Ampr; PT7-aroF3269-pheA3270 | This work |
| pTrcHis2B-AtPAL-FDC1-ppsA–tktA | pBR322 ori; Ampr; PTrc-AtPAL-FDC1-ppsA–tktA | Reference [37] |
| pETDuet-aroFcoli–pheAcoli–ppsA–tktA | ColE1(pBR322) ori; Ampr; PT7-aroFcoli–pheAcoli–ppsA–tktA | This work |
| pACYCDuet-1 | P15A origin; CmR; PT7 | Novagen |
| pACYCduet-ADH2 | P15A origin; CmR; PT7-ADH2 | This work |
| pACYCduet-adh4428 | P15A origin; CmR; PT7-adh4428 | This work |
| pACYCduet-kdc4427–adh4428 | P15A origin; CmR; PT7-kdc4427–adh4428 | This work |
| Strains | ||
| E. coli BL21(DE3) | E. coli B dcm ompT hsdS(rB−mB−) gal | Takara |
| E. coli DH5α | deoR, recA1, endA1, hsdR17(rk−, mk+), phoA, supE44, λ−, thi−1, gyrA96, relA1 | Invitrogen |
| E. coli BL01 | E. coli BL21(DE3) harboring pETDuet-kdc0428 and pACYCDuet-ADH2 | This work |
| E. coli BL02 | E. coli BL21(DE3) harboring pETDuet-kdc0505 and pACYCDuet-ADH2 | This work |
| E. coli BL03 | E. coli BL21(DE3) harboring pETDuet-kdc1244 and pACYCDuet-Adh2 | This work |
| E. coli BL04 | E. coli BL21(DE3) harboring pETDuet-kdc1476 and pACYCDuet-ADH2 | This work |
| E. coli BL05 | E. coli BL21(DE3) harboring pETDuet-kdc3074 and pACYCDuet-ADH2 | This work |
| E. coli BL06 | E. coli BL21(DE3) harboring pETDuet-kdc3075 and pACYCDuet-ADH2 | This work |
| E. coli BL07 | E. coli BL21(DE3) harboring pETDuet-kdc3076 and pACYCDuet-Adh2 | This work |
| E. coli BL08 | E. coli BL21(DE3) harboring pETDuet-kdc3653 and pACYCDuet-ADH2 | This work |
| E. coli BL09 | E. coli BL21(DE3) harboring pETDuet-kdc4427 and pACYCDuet-ADH2 | This work |
| E. coli BL10 | E. coli BL21(DE3) harboring pETDuet-ARO10 and pACYCDuet-Adh4428 | This work |
| E. coli BL11 | E. coli BL21(DE3) harboring pETDuet-ARO10 and pACYCDuet-ADH2 | This work |
| E. coli BL12 | E. coli BL21(DE3) pETDuet-kdc4427 and pACYCDuet-adh4428 | This work |
| E. coli BL13 | E. coli BL21(DE3) harboring pACYCDuet-kdc4427–adh4428 | This work |
| E. coli BL13AP1 | E. coli BL21(DE3) harboring pETDuet-aroFcoli–pheAcoli and pACYCDuet-kdc4427–adh4428 | This work |
| E. coli BL13AP2 | E. coli BL21(DE3) harboring pETDuet-aroFcoli–pheA3272 and harboring pACYCDuet-kdc4427–adh4428 | This work |
| E. coli BL13AP3 | E. coli BL21(DE3) harboring pETDuet-aroFcoli–pheA3270 and pACYCDuet-kdc4427–adh4428 | This work |
| E. coli BL13AP4 | E. coli BL21(DE3) harboring pETDuet-aroG1710-pheAcoli and pACYCDuet-kdc4427–adh4428 | This work |
| E. coli BL13AP5 | E. coli BL21(DE3) harboring pETDuet-aroG1710–pheA3272 and pACYCDuet-kdc4427–adh4428 | This work |
| E. coli BL13AP6 | E. coli BL21(DE3) harboring pETDuet-aroG1710–pheA3270 and pACYCDuet-kdc4427–adh4428 | This work |
| E. coli BL13AP7 | E. coli BL21(DE3) harboring pETDuet-aroH2193-pheAcoli and pACYCDuet-kdc4427–adh4428 | This work |
| E. coli BL13AP8 | E. coli BL21(DE3) harboring pETDuet-aroH2193–pheA3272 and pACYCDuet-kdc4427–adh4428 | This work |
| E. coli BL13AP9 | E. coli BL21(DE3) harboring pETDuet-aroH2193–pheA3270 and pACYCDuet-kdc4427–adh4428 | This work |
| E. coli BL13AP10 | E. coli BL21(DE3) harboring pETDuet-aroF3269–pheAcoli and pACYCDuet-kdc4427–adh4428 | This work |
| E. coli BL13AP11 | E. coli BL21(DE3) harboring pETDuet-aroF3269–pheA3272 and pACYCDuet-kdc4427–adh4428 | This work |
| E. coli BL13AP12 | E. coli BL21(DE3) harboring pETDuet-aroF3269–pheA3270 and pACYCDuet-kdc4427–adh4428 | This work |
| E. coli BL13TP1 | E. coli BL21(DE3) harboring pETDuet-tktA–ppsA and pACYCDuet-kdc4427–adh4428 | This work |
| E. coli BL13TP2 | E. coli BL21(DE3) harboring pETDuet-tktA–ppsA–aroFcoli–pheAcoli and pACYCDuet-kdc4427–adh4428 | This work |
| E. coli LCQ-1 | E. coli BL21(DE3) harboring pETDuet-kdc4427 | This work |
| E. coli LCQ-2 | E. coli BL21(DE3) harboring pETDuet-ARO10 | This work |
| E. coli LCQ-3 | E. coli BL21(DE3) harboring pETDuet-adh4428 | This work |
| E. coli LCQ-4 | E. coli BL21(DE3) harboring pETDuet-ADH2 | This work |