Fig. 3.

Effects of angiotensin II and/or irbesartan on VEGF mRNA expression in ASCs under normoxia (A) and hypoxia (B). Real-time RT-PCR analysis was performed for determination of VEGF mRNA expression levels relative to those of β-actin mRNA, which are expressed as the ratio to the control value (Control, gray bar) for ASCs treated with 100 nM angiotensin II alone (AngII, blue bar), 200 nM irbesartan alone (Irb, yellow bar), and both 100 nM angiotensin II and 200 nM irbesartan (AngII + Irb, green bar) (n = 3 each). **P < 0.01, *P < 0.05 (vs. Control).