Fig. 7.

Culture results of human iPS cells. Human induced pluripotent stem cells (hiPSCs) were passaged both manually and by the Tissue Factory. hiPSCs cultured by the Tissue Factory showed comparable growth ratios and characteristics to hiPSCs cultured manually. A) Microscopic view of hiPSCs passaged manually. B) Microscopic view of hiPSCs passaged in the Tissue Factory. C) Growth rates through three successive passages. D) Live cell ratios measured using Trypan Blue dye. E) Flow cytometric analysis of hiPSCs after three passages by stage specific embryonic antigen 4 (SSEA4) and rBC2LCN lectin. Outlined histograms shows isotype control and no-dye control in SSEA4 and rBC2LCN, respectively. Filled histograms show the measured data. Horizontal axes indicate the fluorescent intensities of fluorescein isothiocyanate (FITC) conjugated with SSEA4 and rBC2LCN. Vertical axes indicate cell numbers at each intensity. Percentages of positive (undifferentiated) cells are annotated by the thresholds where the control samples were measured as 0.5%.