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. 2018 Nov 7;18:296. doi: 10.1186/s12906-018-2353-z

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© The Author(s). 2018

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Western blotting analyses of aloe-emodin on the expression of apoptotic proteins. a SCC15 cells were treatment with various concentrations (0, 25 and 50 μM) of aloe-emodin for 48 h and the levels of caspase-9, caspase-3 were measured by Western blotting analysis. Increased expression of caspase-9 and caspase-3 were observed. β-actin was used as a loading control. b Densitometry analysis of Western blots, Data were expressed as the mean ± SD (n = 3) and analyzed by one-way ANOVA using Dunnett’s multiple-comparison test (*P < 0.05, **P < 0.01)