Fig. 4.

Comparison of the performance of isoform quantification tools on BLUEPRINT B lymphocyte bulk and single-cell RNA-seq data. Each point represents one cell from the scRNA-seq dataset or one bulk RNA-seq experiment. Yellow points represent bulk RNA-seq experiments, blue points represent one cell from the scRNA-seq experiment. a F1 score and precision and recall of isoform detection. The F1 score is the harmonic mean of the precision and recall. The precision is the proportion of the isoforms predicted to be expressed by an isoform quantification tool which are expressed. The recall is the proportion of expressed isoforms which are predicted to be expressed by the isoform quantification tool. b Spearman’s rho. c Normalized root mean square error (NRMSE)