Fig. 6.

Investigation into how many isoforms are expressed per cell in the scRNA-seq data for genes which express exactly two isoforms in bulk data. The BLUEPRINT B lymphocyte (left) and the Kolodziejczyk et al. ES cell (right) bulk RNA-seq data are shown. The B lymphocyte graphs shown here are from the first biological replicate of the BLUEPRINT male B lymphocyte bulk RNA-seq; equivalent graphs for the second and third BLUEPRINT male B lymphocyte biological replicates can be found in Additional file 1: Figure S15. a Number of genes which express two isoforms in the bulk RNA-seq data expressing zero, one or two isoforms in each cell in the scRNA-seq data. b Histogram of the percentage of cells which express both the isoforms detected in the bulk RNA-seq data. c Relationship between the percentage of cells which express both the isoforms detected in the bulk RNA-seq. Spearman’s rho is 0.623 for the BLUEPRINT B lymphocytes and 0.795 for the Kolodziejczyk et al. ES cells