Table 1.
Primary antibodies
| Immunogen | Host species | Manufacturer | Catalog # | Application | Dilution |
|---|---|---|---|---|---|
| Lcn2 (rat) | goat | RD Systems | AF3508 | IB | 1:200-300 |
| Lcn2 (mouse) | rabbit | Abcam | Ab63929 | IF | 1:250-500 |
| Lcn2-R (C-term) (rat) | rabbit | Immunoglobe | Ig-1086 | IB | 1:250-2,000 |
| Lcn2-R (N-term) (rat) | rabbit | Immunoglobe | Ig-1095 | IF | 1:100-1000 |
| β-actin | mouse | Sigma-Aldrich | A5316 | IB | 1:20,000 |
| β-catenin (mouse) | mouse | BD Biosciences | 610153 | IB | 1:2,000-5,000 |
| GSK-3β (rat) | mouse | BD Biosciences | 610201 | IB | 1:500 |
| Phospho-GSK-3 β (Ser9) (human) | rabbit | Cell Signaling Technology | 9336 | IB | 1:500 |
| Na+,K+-ATPase α1-subunit (human) | rabbit | Cell Signaling Technology | 3010S | IB IF |
1:250-500 1:100 |
| FLAG-M2 | mouse | Sigma-Aldrich | F3165 | IB | 10μg/ml |
IB = immunoblotting. IF = immunofluorescence