Table 1.
Demographics and clinical characteristics of ALS (A,B,C) and healthy control (D) individuals included in the discovery proteomics (A, B) and in the re-test immunoassays (C, D)
| A. DISCOVERY EXPERIMENT: ALS PLASMA SAMPLES | |||||||||
| ALS type |
ALSFRS-R early |
ALSFRS-R late |
Gender M/F |
Age at onset (years) | PRL | Time from onset to death or last visit (months) | Time from onset to first sample (months) | Time between first and last sample (months) | Site of disease onset limb/ bubar |
| Slow (n = 6) | 43 (41–45) |
31.5 (27–35) |
5 M / 1F | 58.1 (35–71) | 0.25 (0.1–0.4) |
107 (97, 254) |
76 (30–192) |
58 (48–69) |
4 limb /2 bulbar |
|
Fast
(n = 6) |
43.8 (41–46) |
28.6 (22–35) |
3 M / 3F | 61.3 (48–67) | 1.5 (1–1.9) | 20.5 (11–32) | 10.2 (5–19) |
13.8 (7–22) | 3 limb /3 bulbar |
| B. DISCOVERY EXPERIMENT: ALS PBMC SAMPLE TO FORM THE REFERENCE POOL | |||||||||
| ALS Pool |
ALSRS-R | Gender M/F |
Age at onset (years) | PRL | Time from onset to death or last visit (months) | Time from onset to sampling (months) | Site of disease onset limb/ bubar | ||
|
Slow(3) Fast (2) n = 5 |
31 (22–42) |
3 M /2 F | 65.8 (57–68) | 0.8 (0.02–2.5) |
64.6 (8–120) |
19 (7–44) |
3 limb /2 bulbar | ||
| C. RE-TEST EXPERIMENT: ALS PLASMA SAMPLES | |||||||||
| ALS type | ALSRS-R | Gender M/F |
Age at onset (years) | PRL | Time from onset to death or last visit (months) | Time from onset to sampling (months) | Site of disease onset limb/ bubar | ||
|
Slow
(n = 23) |
39 (18–47) |
18 M / 5F | 65.25 (37–86.9) | 0.216 (0.03–0.47) | 120.4 (28–335.2) |
62.8 (7–248.8) |
15 limb /8 bulbar | ||
|
Fast
(n = 24) |
33 (13–43) |
8 M / 16F | 62.8 (34.8–82.4) | 1.48 (0.74–3.6) | 28.1 (5–50) |
14.8 (2.8–29.8) |
13 limb /11 bulbar | ||
| D. RE-TEST EXPERIMENT: HEALTHY CONTROLS PLASMA SAMPLES | |||||||||
| n = 29 | Gender M/F | Age at sampling (years) | |||||||
| 8 M / 17F | 60.9 (50.8–73) | ||||||||