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Determination of the correct axial slice position at the level of the lower endplate of L3 vertebra and skeletal muscle fat content by PDFF. Iliac crest tangent sign on coronal images, marking either L4 vertebra or L4/5 intervertebral disc and cross-reference to axial images for the identification of level L3 vertebra (a and b). Mean skeletal muscle fat content as mean PDFF in one ROI at level L3 vertebra (b). PDFF, proton-density fat fraction; ROI, region of interest.
