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. 2018 Oct 10;8(10):180086. doi: 10.1098/rsob.180086

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© 2018 The Authors.

Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.

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Figure 1. — IDH1 R132H mutation induces proliferation and migration of NSCLC cells. (a) WB analysis of IDH1 and IDH1 R132H protein levels in H1299 cells transduced with an empty vector, IDH1 WT or mutant IDH1 R132H. (b) Proliferation of H1299 cells transduced with an empty vector, IDH1 WT or mutant IDH1 R132H assessed by using the MTS assay. (c) Migration of H1299 cells transduced with an empty vector, IDH1 WT or mutant IDH1 R132H was assessed by Transwell migration assay. (d) WB of IDH1 protein in H460 cells after transfection with IDH1 siRNA. (e) Proliferation of H460 cells after transfection with IDH1 siRNA was assessed by MTS assay. (f) Migration of H460 cells after transfection with IDH1 siRNA was assessed by Transwell migration assay. (g) Proliferation of H1299 cells expressing mutant IDH1 R132H and treated with 20 µM IDH1 inhibitor (AGI-5198) was assessed by MTS assay. (h) Migration of H1299 cells treated as in (g) was assessed by Transwell migration assay.