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. 2018 Oct 10;8(10):180086. doi: 10.1098/rsob.180086

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© 2018 The Authors.

Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.

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Figure 3. — IDH1 mutation induces methylation of Fibulin-5 promoter. (a) mRNA levels of fibulin proteins in H1299 cells treated with 2 mM 2-HG. (b) Genomic PCR of unmethylated promoters of Fibulin-1 and Fibulin-5 genes in H1299 cells treated with 2 mM 2-HG. (c) Genomic PCR of unmethylated promoters of Fibulin-5 gene in H460 and H1299 cells. (d) WB analysis of Fibulin-5 in H1299 and H460 cells. (e) Fibulin-1 and Fibulin-5 mRNA levels in H460 cells transduced with IDH1 siRNA. (f) Fibulin-1 and Fibulin-5 mRNA levels in H1299 cells expressing an empty vector, IDH1 WT or mutant IDH1 R132H.