Figure 3.

IL-38 inhibits β-catenin expression in NSCLC cells. (a) The mRNA level of β-catenin was measured by qPCR. A549 and SK-MES-1 cells were treated with 100 ng ml⁻¹ rhIL-38. n = 3. *p < 0.05. (b) The protein level of β-catenin was measured by western blot. A549 and SK-MES-1 cells were treated with 100 ng ml⁻¹ rhIL-38. n = 3. *p < 0.05. (c–g) The wound healing, cell invasion, apoptosis and survival rates and the clone-forming ability of cells overexpressing β-catenin or empty vector were analysed. Cells were treated with 100 ng ml⁻¹ rhIL-38. n = 3. *p < 0.05.