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. 2018 Oct 10;8(10):180118. doi: 10.1098/rsob.180118

Figure 3.

Figure 3.

Demethylation and activation of endogenous miR-125a-5p through exogenous over-expressing miR-125a-5p. (a) Over-expressing miR-125a-5p could suppress the H3K9me3 level in NSCLC cell lines (A549 and SK-MES-1), determined by western blot. (b) Over-expressing miR-125a-5p could suppress the miR-125a-5p methylation levels in NSCLC cell lines (A549 and SK-MES-1). n = 3. *p < 0.05. (c) Over-expressing miR-125a-5p could upregulate the endogenous miR-125a-5p precursor expression in NSCLC cell lines (A549 and SK-MES-1), determined by qPCR. n = 3. *p < 0.05. (d) Successful over-expression of Suv39H1, determined by western blot in NSCLC cell lines (A549 and SK-MES-1). (e) Upregulation of endogenous miR-125a-5p precursor by exogenous miR-125a-5p over-expression could be abolished by Suv39H1 over-expression in NSCLC cell lines (A549 and SK-MES-1), determined by qPCR. n = 3. *p < 0.05. (f) The demethylation of miR-125a-5p by exogenous miR-125a-5p over-expression could be abolished by Suv39H1 over-expression in NSCLC cell lines (A549 and SK-MES-1). n = 3. *p < 0.05. NC: empty vector.