Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Oct 21;9:175–191. doi: 10.1016/j.isci.2018.10.019

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2018 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

GGPP Is Necessary to Modulate Apoptosis in Adipocytes

(A–C) After the primary WAT cells were differentiated and kept until D4, they were treated with 30 μM Zol in combination with or without FPP or GGPP for an additional 4 days (A). The lipids and nuclei in primary WAT cells were stained with Nile red (top panel) and Hoechst 33342 (middle panel), respectively (A). TG accumulation levels (B) and cell viability (C) are also shown. Merge (bottom panel), the combined images of Nile red and Hoechst. Scale bars, 200 μm.

(D and E) Annexin V (top panel) and PI (middle panel) staining (D) and measurement of apoptosis-related gene expression (E) were performed in primary WAT at D8. Merge (bottom panel), the combined images of Annexin V and PI. Scale bars, 200 μm.

(F and G) Annexin V (top panel) and PI (middle panel) staining (F) and measurement of apoptosis-related gene expression (G) were performed in coxsackievirus-adenovirus receptor-overexpressed 3T3-L1 infected with shlacZ or shGGPS#2 at D8. Merge (bottom panel), the combined images of Annexin V and PI. Scale bars, 200 μm.

Bars represent mean ± SE. (n = 6–8). Bars with different letters represent significant differences (p < 0.05) by one-way ANOVA with a post-hoc Tukey HSD test.