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. Author manuscript; available in PMC: 2018 Nov 8.
Published in final edited form as: Cell Rep. 2018 Jun 26;23(13):3741–3749. doi: 10.1016/j.celrep.2018.05.084

Figure 3. PARP1 Suppresses Expression of Siah1 at Both mRNA and Protein Levels.

Figure 3.

(A–C) ELL2 mRNA isolated from HeLa cells expressing shNT or shPARP1 (A), from WT and PARP1 KO HeLa cells (B), and from HeLa cells treated with DMSO or AZD2281 (C) were measured using qRT-PCR, normalized to those of GAPDH, and displayed, with the value of the first column in each panel set to 1. Error bars represent mean ± SD from three separate measurements.

(D) WCE of HeLa cells expressing the indicated WT and mutant Flag-ELL2 and treated with DMSO or AZD2281 were examined using WB.

(E and F) The indicated mRNA isolated from HeLa cells expressing shNT or shPARP1 (E) or from WT and PARP1 KO HeLa cells (F) were measured using qRT-PCR, normalized to those of GAPDH, and displayed, with the values obtained in the shNT and WT cells adjusted to 1. Error bars represent mean ± SD from three separate measurements.

(G and H) Endogenous Siah1 (G), Flag-Siah1 expressed from a transfected plasmid (H), and other indicated proteins present in NE (G) or WCE (H) of control and PARP1 KD (shPARP1) cells were examined using WB.