Figure 5.

HOTAIR promotes autophagy by upregulating ATG7 expression.

Notes: (A, B) The mRNA expression of several autophagy-related genes in PANC-1 and AsPC-1 cells transfected with pcDNA3.1-HOTAIR were measured by real-time PCR. (C, D) ATG7 protein from the PANC-1 and AsPC-1cells was detected by Western blotting, in which cells were transfected with pcDNA3.1-HOTAIR compared with negative control plasmid. (C, D) ATG7 protein from the PANC-1 and AsPC-1 cells was detected by Western blotting, in which cells were transfected with si-HOTAIR compared with negative control plasmid. *P<0.05.

Abbreviations: ATG7, autophagy-related gene 7; HOTAIR, HOX transcript antisense RNA.