Table 1.
Strains and plasmids used in this work
| Strain or plasmid | Relevant characteristic(s) | Reference or source |
|---|---|---|
| Strain | ||
| Escherichia coli | ||
| DH5α | Es. coli DH5α used as a host for the PCR products cloning of the resistance genes | Our laboratory collection |
| ATCC 25922 | Es. coli ATCC 25922 used as the quality control for the antimicrobial test | Our laboratory collection |
| Enterococcus faecalis | ||
| JH2-2 | En. faecalis JH2-2 used as the host for the resistance genes cloning and the recipient for the conjugation experiment, Rfr | Our laboratory collection |
| ATCC 29212 | En. faecalis ATCC 29212 used as the quality control strain for the antimicrobial test | Our laboratory collection |
| EC369 | The wild strain of Enterococcus casseliflavus 369 | This study |
| Es. coli carrying plasmid | ||
| pUCP20-ORFs/DH5α | DH5α carrying the recombinant plasmids pUCP20-ORFs (ant6, aph3′, ermB, and sat4) | This study |
| pUCP24-ORF/DH5α | DH5α carrying the recombinant plasmid pUCP24-ORF (bla) | This study |
| En. faecalis carrying plasmid | ||
| pAM401/JH2-2 | JH2-2 carrying vector pAM401, CHLr | Our laboratory collection |
| pAM401-ORFs/JH2-2 | JH2-2 carrying the recombinant plasmids of pAM401 cloned with resistance gene ORFs with promoter regions (ant6, aph3’, bla, sat4, ermB) | This study |
| Plasmid | ||
| pUCP24 | Cloning vector for the PCR products of bla gene, GMr | Our laboratory collection |
| pUCP20 | Cloning vector for the PCR products of ant6, aph3’, ermB, and sat4, Apr | Our laboratory collection |
| pAM401 | Cloning vector for the PCR products of all resistance genes with the promoter regions, CHLr | Our laboratory collection |
Abbreviations: CHL, chloramphenicol; GM, gentamicin; ORFs, open reading frames
r
resistance; RF, rifampin; AP, ampcillin.