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Effect of infiltration medium on transient GUS expression in coffee leaf disks injected with A. tumefaciens GV3101::pCAMBIA 1303. a GUS staining observed 48 h after infiltration. b Percentage of GUS-stained area detected using Image J measurements. Each value represents the mean ± SD of fifteen repetitions. Means with the same letter are not significantly different (Duncan test p = 0.05). Bar: 1 cm
