Fig. 6.

a Expression of the uidA gene 5 weeks after infiltration. PCR analysis showing the presence of the genes b mgfp5 (amplicon size 790 bp) and c uidA (amplicon size 710 bp) and the absence of the gene d virG in agroinfiltrated coffee leaf disks (amplicon size 390 bp). M molecular weight marker (1 Kb Plus, Fermentas), NTC negative control (PCR reaction mix without template), P+ positive control (pCAMBIA 1303 DNA), C- wildtype leaf disks (non-injected control), 1 and 2: putative transgenic coffee leaf disks. Bar: 1 cm