Fig. 2.

Effect of HCD on dermal adipose remodeling and inflammation. a Representative photomicrographs of immunohistochemical sections from indicated groups following staining with hematoxylin and eosin, F4/80 or anti-IL1-beta. (Scale bars: 500 µm (low mag) and 25 µm (high mag).) b Thickness of dermal adipose tissue was determined relative to the combined papillary dermis and reticular dermis thickness. For each of five mice, 10 randomly selected measurements were calculated from histological images and measured using image J. Data represents mean ± SEM (n = 5 in each group) *p < 0.05, **p < 0.01, ***p < 0.001 vs. age-matched control diet fed mice. c Effects of age and HCD on COX-2 expression in mouse skin