Skip to main content
. Author manuscript; available in PMC: 2019 Oct 4.
Published in final edited form as: Mol Cell. 2018 Sep 13;72(1):48–59.e4. doi: 10.1016/j.molcel.2018.08.009

Figure 1.

Figure 1.

CRTH2 ligands and overall structures. (A) Chemical structures of PGD2, fevipiprant, CAY10471 and ramatroban and the conserved carboxylate group. (B) Competition radioactive ligand binding assays with HEK-293T cell membranes expressing wtCRTH2 and CRTH2-mT4L. For each experiment, 2 nM [3H] PGD2 was used and various concentrations of PGD2 (top), CAY10471 (middle) and fevipirant (bottom) were added as competing ligands. Data points are presented as the mean values +/− SEM, n=3. (C) Overall structures of fevipiprant-bound and CAY10471-bound CRTH2 are colored in blue and slate, respectively. Fevipiprant and CAY10471 are shown as orange and yellow spheres.