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. Author manuscript; available in PMC: 2019 Sep 15.
Published in final edited form as: Neuropharmacology. 2018 Aug 4;140:150–161. doi: 10.1016/j.neuropharm.2018.08.003

FIGURE 7:

FIGURE 7:

Effects of GW0742 on apoptosis via the PPAR-β/δ/TXNIP/Trx-1/p-ASK1/p-p38 signaling pathway at 72 hours post hypoxia-ischemia (HI). (A) Representative picture of western blot data showing bands of the expression levels of TXNIP, thioredoxin-1 (Trx-1), p-ASK1, p-p38, Bcl-2, Bax and cleaved caspase-3 either with GW0742 treatment alone or GW0742+DMSO in corn oil, GW0742+GSK3787, GW0742+control CRISPR, GW0742+TXNIP CRISPR, GW0742+control LNA antimir, GW0742+antimir-17-5p groups. (B–G) Western blot data quantification of bands showed that GW0742 significantly increased Trx-1 and Bcl-2/Bax ratio and significantly decreased TXNIP, p-ASK1, p-p38 and caspase-3 cleavage when compared with HI+vehicle. GSK3787, TXNIP CRISPR activation plasmid and antimir-17-5p showed to significantly increase TXNIP (B), p-ASK1 (D), p-p38 (E) and cleavage of caspase-3 (G) expression, but decrease Trx-1 (C), Bcl-2/Bax (F) expression when compared with GW0742+DMSO in corn oil, GW0742+control CRISPR and GW0742+control LNA groups, respectively. *p < 0.05 vs. sham; #p < 0.05 vs. HI+ vehicle; &p < 0.05 vs. HI+GW0742+DMSO in corn oil; @p < 0.05 vs. HI+GW0742+ control CRISPR; $p < 0.05 vs. HI+GW0742+control LNA. n = 6 for each group. n=5 for HI+GW0742+antimir-17-5p.