Fig. 5.

Mucosal antibody response against OVA at different mucosal sites. Mice were orally immunized with 100 µg OVA alone, 5 mg treated ragweed (RW) pollen alone, or 100 µg OVA and 5 mg RW every week for eight weeks. Anti-OVA IgA response in (A) fecal samples, (B) saliva, (C) vaginal wash, and (D) BAL fluid (day 160). Each symbol represents the response from an individual mouse (n = 10 for fecal, saliva, and vaginal wash; n = 7 for BAL fluid). Feces, saliva, and vaginal secretions were diluted in PBST at 1:5 dilution while BAL fluids were diluted at 1:2 dilution in PB T. p ≤ 0.05 [*] p ≤ 0.01 [**] (horizontal bar represents the group mean)