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. Author manuscript; available in PMC: 2019 Sep 1.
Published in final edited form as: Regen Eng Transl Med. 2018 Apr 23;4(3):120–132. doi: 10.1007/s40883-018-0054-2

Fig. 3. Lymphatic and blood vessel structures in a collagen matrix.

Fig. 3

(A, B) LA and VA were induced by the addition of VEGF-C (10 ng/ml). HUVEC started to migrate and sprout into collagen gel at D3, prior to LEC sprouting. (C) Quantitative comparison of sprouting length (D) and effective diameter (E) of HUVEC and LEC. n=12. LA lagged behind VA. (F) Immunostaining of podoplanin (red) and F-actin (green) and nuclear (blue) of LA and VA at D7 at 10 and 50 ng/ml of VEGF-C. (G) Enhancement of LA and VA at D7 in a dose-dependent manner of VEGF-C (1, 5, 10 and 50 ng/ml). n=5. (H) Single cell migration of HUVEC and LEC at D7. n=5. *, p<0.01; **, p<0.05, error bar=±SD.