Fig. 5.

EV-associated, V1S-tagged α-syn is readily taken up by recipient cells. a Example images from cells receiving EV or FFP fractions from cells expressing V1S + SV2 (BiFC) or V1S only. Conditioned medium was added to non-transfected, SHSY5Y recipient cells for 24 h. The cells were washed twice in PBS and, following fixation, immunocytochemistry against the hemi-Venus tag was performed to allow visualization and display both constructs independently of dimerization (green). b The EV-associated V1S-tagged α-syn was taken up or retained to a significantly greater extent than the other fractions. Dark purple bars represent the EV fractions and the light purple bars represent the FFP fractions (mean ± SD, n = 4). One-way ANOVA with Tukey’s post hoc test was used to determine the differences between the groups (*p < 0.05, **p < 0.01, ***p < 0.001)