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. 2018 Oct 4;38(8):1539–1550. doi: 10.1007/s10571-018-0622-5

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 6 — A53T α-syn was enriched in the EV fraction. a Absolute levels of α-syn in EV (light green: RIPA–, dark green: RIPA+), b FFP and c IC fractions, as measured by ELISA. d Percentage of α-syn in EV compared to FFP fractions. e Percentage of α-syn in EV compared to IC fractions. f Percentage of α-syn in FFP compared to IC fractions. The distribution of A53T differed from WT α-syn and the other mutants and was higher in both RIPA+ and RIPA− fractions when normalized against IC values. Bars represent mean ± SD, n = 3. Differences between the respective RIPA− and RIPA+ fractions from WT α-syn were calculated by one-way ANOVA with Dunnett’s post hoc test (*p < 0.05, **p < 0.01, ***p < 0.001)