Fig. 6.

(a) IBA1-immunolabelled microglia (green) in retinal sections. Microglia demonstrated both dendritic and amoeboid morphologies; scale bar, 50 μm. (b, c) Scatter plots showing total microglial density (b) and the density of amoeboid microglia (c) in sections from control animals (circles), animals with diabetes alone (diamonds) and diabetic animals (triangles) treated with 2-HDP; n=6 retinas from six animals in each experimental group. (d–f) Column scatter graphs summarising retinal mRNA expression for the inflammatory signalling molecules Il1b (d), Ccl2 (e) and Icam1 (f), expressed as a ratio of expression in control tissues. Circles, control; diamonds, diabetes; triangles, diabetes + 2-HDP; n=6 control and diabetes + 2-HDP retinas from six animals; n=7 diabetic retinas from seven animals. *p<0.05, **p<0.01 and ***p<0.001 for the indicated comparisons. GCL, ganglion cell layer; INL, inner nuclear layer; IPL, inner plexiform layer; ONL, outer nuclear layer; OPL, outer plexiform layer; PL, photoreceptor layer