Fig. 5.

miR-200b targets ZEB1. ZEB1 mRNA levels (a) in HGF were measured by real-time PCR. GAPDH was used as an internal normalization control. ZEB1 protein levels (c) were determined by Western blotting. HGF were transfected with miR-200b expression plasmid or control plasmid, and treated with or without IL-1β (1 ng/ml) or TNF-α (10 ng/ml) for 24 h. miRanda and TargetScanHuman (6.2) showed that Zeb1 3′-UTR contains several putative miR-200b target sites (b1–5)