Fig. 5.

NDAT inhibits PI3K activity in colorectal cancer cells. Colorectal cancer HCT116 cells were seeded and grown on sterilized cover glass slides and then were treated with a PI3K inhibitor (LY294002, 10 μM) with or without gefitinib (1 μM) for 24 h. The cells were immediately fixed and FITC-SNA was applied to bind to α2,6-linked sialic acid structure. Confocal microscopy was used to examine ST6Gal1-catalyzed α2,6 sialylation (green color) and EGFR expression (red color) in colorectal cancer cells