Table 1.
Settings for real-time PCR and primers at different institutions
| Facility | DNA extraction | Real-time PCR | CMV amplicon | Primers and probe |
|---|---|---|---|---|
| A | EZ1 Advanced XL EZ1 Virus Mini (Qiagen) |
Light cycler 2.0 (Roche) | IE-1 | CATGAAGGTCTTTGCCCAGTAC GGCCAAAGTGTAGGCTACAATAG Probe TGGCCCGTAGGTCATCCACACTAGG |
| B | QIAamp Viral RNA Mini (Qiagen) | ABI 7000 (Applied Biosystems) |
DNA polymerase | GCTGACGCGTTTGGTCATC ACGATTCACGGAGCACCAG Probe FAM-TCGGCGGATCACCACGTTCG-TAMRA |
| C | QIAamp DNA Mini (Qiagen) | StepOnePlus (Applied Biosystems) |
DNA polymerase | GCTGACGCGTTTGGTCATC ACGATTCACGGAGCACCAG Probe FAM-TCGGCGGATCACCACGTTCG-TAMRA |
| D | DNA Extract All (Applied Biosystems) |
StepOnePlus (Applied Biosystems) |
Pa034534000_s1 Applied biosystems |
|
| E | QIAamp DNA Mini Kit (Qiagen) | Light cycler 2.0 (Roche) | Glycoprotein B | AAGTACCCCTATCGCGTGTG ATGATGCCCTCRTCCARGTC |