Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Aug 13;470(12):1739–1751. doi: 10.1007/s00424-018-2190-4

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

PMC Copyright notice

Fig. 1 — GLUT9 is mainly expressed at the basolateral membrane of the DCT and slightly in the PCT. a Western blot of microdissected tubules from wild-type mice. GLUT9 is strongly detectable in the DCT. Some expression is also visible in the PCT after a longer exposure. NCX1 is used as positive control for the accuracy of the DCT microdissection. Protein loading can be evaluated by actin quantification. b Immunostaining of GLUT9 on wild-type kidney section. GLUT9 signal is restricted to cortical distal convoluted tubules (scale bar: 100 μm). c Co-immunostaining of GLUT9 and NCX1, and of GLUT9 and AQP2 on wild-type kidney sections. Both GLUT9 and NCX1 signals are co-localizing at the basolateral membrane of the DCT. There is no co-localization of GLUT9 and AQP2 (scale bar: 10 μm)