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. 2018 Nov 8;13(11):e0205983. doi: 10.1371/journal.pone.0205983

Fig 3. E. chaffeensis TRP47 binds host gDNA via the tandem repeat domain.

Fig 3

(A) TRP47 5 tandem repeat (5TR) construct binds to host genomic DNA in EMSA. Incubation of biotin-labeled host gDNA with 4 μg TRP47 5TR (lane 2) yielded a strong shifted band (band height marked by arrow) compared to labeled gDNA alone (lane 1). Binding was prevented with addition of excess unlabeled competitor gDNA (lane 3), and with high concentration of anti-TRP47 antibody (lanes 4–7 are with 1:20, 1:50, 1:100, and 1:200, respectively). These lanes were rearranged from the original blot and white lines denote lanes that were not adjacent. (B) TRP47 full-length (FL) construct binds to host genomic DNA in EMSA. Incubation of labeled gDNA with 8 μg TRP47 FL (lane 3) yielded a shifted band (marked by arrow) not present with labeled gDNA alone (lane 1) nor with 4 μg TRP47 FL (lane 2). Binding was prevented with addition of excess unlabeled competitor gDNA (lane 4). A different gDNA preparation was used for the EMSA in panel B likely accounting for the difference in free gDNA migration and background.